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How to Time Multi-Step Lab Protocols

A simple workflow for running multi-step lab protocols with synced timers so each step stays on track.

Running a multi-step protocol—PCR, HPLC, cell culture, or anything with repeated timing—means keeping several timers in your head at once. Here’s a straightforward way to do it with Lab Laps.

1. Create a project per protocol

Create one project per protocol (e.g. “qPCR run” or “Western blot”). That keeps steps and timers grouped and easy to reuse.

2. Add a step for each phase

Add a step for each phase of the protocol: denaturation, annealing, extension, incubation, washes, etc. Use the step name and description to note any specifics (temperatures, volumes, tips).

3. Add timers to the first step

In the first step, add one timer per thing you need to track: e.g. “Denature 30s”, “Anneal 30s”, “Extend 45s”. Set the correct durations.

4. Turn on timer sync

Enable Sync timers in the project settings. The same timers are then shared across all steps. Start them once in step 1; when you move to step 2, 3, 4… the elapsed time stays in sync. No need to restart or remember where you left off.

5. Use groups for long protocols

For long protocols with clear blocks (e.g. “Block 1: PCR”, “Block 2: Gel”), add groups and put the relevant steps inside. You can collapse groups to focus on the block you’re in.


Lab Laps keeps experiments organized with protocol-ready timing, autosave, and optional cloud sync. Create a project and try it on your next run.